packaging line Search Results


retroviral packaging cell line  (ATCC)
92
ATCC retroviral packaging cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Retroviral Packaging Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/retroviral packaging cell line/product/ATCC
Average 92 stars, based on 1 article reviews
retroviral packaging cell line - by Bioz Stars, 2026-06
92/100 stars
  Buy from Supplier
phoenixeco ecotropic packaging cell line  (BioWhittaker Molecular Applications)
90
BioWhittaker Molecular Applications phoenixeco ecotropic packaging cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Phoenixeco Ecotropic Packaging Cell Line, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phoenixeco ecotropic packaging cell line/product/BioWhittaker Molecular Applications
Average 90 stars, based on 1 article reviews
phoenixeco ecotropic packaging cell line - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
phoenixtm eco retroviral packaging cell line  (Orbigen Inc)
90
Orbigen Inc phoenixtm eco retroviral packaging cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Phoenixtm Eco Retroviral Packaging Cell Line, supplied by Orbigen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phoenixtm eco retroviral packaging cell line/product/Orbigen Inc
Average 90 stars, based on 1 article reviews
phoenixtm eco retroviral packaging cell line - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
gp+e86 packaging cell line  (Genetix Pharmaceuticals Inc)
90
Genetix Pharmaceuticals Inc gp+e86 packaging cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Gp+E86 Packaging Cell Line, supplied by Genetix Pharmaceuticals Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gp+e86 packaging cell line/product/Genetix Pharmaceuticals Inc
Average 90 stars, based on 1 article reviews
gp+e86 packaging cell line - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
φnx packaging cell line  (Orbigen Inc)
90
Orbigen Inc φnx packaging cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
φnx Packaging Cell Line, supplied by Orbigen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/φnx packaging cell line/product/Orbigen Inc
Average 90 stars, based on 1 article reviews
φnx packaging cell line - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
ecopack2–293  (Becton Dickinson)
90
Becton Dickinson ecopack2–293
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Ecopack2–293, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecopack2–293/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
ecopack2–293 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
high pressure’s fruit jelly  (Meidi ya Coporation)
90
Meidi ya Coporation high pressure’s fruit jelly
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
High Pressure’s Fruit Jelly, supplied by Meidi ya Coporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high pressure’s fruit jelly/product/Meidi ya Coporation
Average 90 stars, based on 1 article reviews
high pressure’s fruit jelly - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
the 293t cell line  (Orbigen Inc)
90
Orbigen Inc the 293t cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
The 293t Cell Line, supplied by Orbigen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/the 293t cell line/product/Orbigen Inc
Average 90 stars, based on 1 article reviews
the 293t cell line - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
retroviral packaging cells, line pa317  (Promega)
90
Promega retroviral packaging cells, line pa317
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Retroviral Packaging Cells, Line Pa317, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/retroviral packaging cells, line pa317/product/Promega
Average 90 stars, based on 1 article reviews
retroviral packaging cells, line pa317 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
packaging cell line c2  (ICN Biomedicals)
90
ICN Biomedicals packaging cell line c2
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Packaging Cell Line C2, supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/packaging cell line c2/product/ICN Biomedicals
Average 90 stars, based on 1 article reviews
packaging cell line c2 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
phoenix-eco 293 packaging cell line  (Orbigen Inc)
90
Orbigen Inc phoenix-eco 293 packaging cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Phoenix Eco 293 Packaging Cell Line, supplied by Orbigen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phoenix-eco 293 packaging cell line/product/Orbigen Inc
Average 90 stars, based on 1 article reviews
phoenix-eco 293 packaging cell line - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
statistics package ldp-line  (CH Instruments)
90
CH Instruments statistics package ldp-line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Statistics Package Ldp Line, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/statistics package ldp-line/product/CH Instruments
Average 90 stars, based on 1 article reviews
statistics package ldp-line - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a retroviral vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.

Journal: Virology

Article Title: Characterization of the Epstein-Barr virus glycoprotein BMRF-2.

doi: 10.1016/j.virol.2006.09.047

Figure Lengend Snippet: Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a retroviral vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.

Article Snippet: A human embryonic kidney cell line (293T) and a retroviral packaging cell line (ProPak A.52) were purchased from American Type Culture Collection (ATCC,Manassas, Virginia).

Techniques: Glycoproteomics, Transduction, Retroviral, Plasmid Preparation, Expressing, Staining, Fluorescence, Transfection, Membrane, Isolation, Purification, Control, Western Blot